69250:SMOOTH MUSCLE ANTIBODY

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69250:SMOOTH MUSCLE ANTIBODY
Alias Names: ANTI-SMOOTH MUSCLE ANTIBODY
Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Edit Date: 4/1/2005
Performed: Thursday
Released: Same day as tested
CPT Code: 83516
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling: Allow to clot 10 minutes. Centrifuge and separate serum from cells immediately and pour into a plastic vial. Refrigerate until transported. Freeze at -20° C if specimen cannot be assayed within 5 days. Avoid repeated freeze/thaw cycles.
Standard Volume: 1 mL serum.
Minimum Volume: 100 µL serum.
Transport: Refrigerated; or if frozen, on dry ice.
Rejection Criteria: Plasma; gross lipemia; hemolysis; contaminated specimen.

Smooth Muscle antibody, IgG Result Interpretation ELISA Method:

<20.0 EU (EU=ELISA Unit)

<20.0 EU 20.0-30.0 EU >30.0 EU
Negative: No Smooth Muscle antibody (ASMA) detected. Weak Positive: Repeat testing may be helpful. Positive: Smooth Muscle, IgG antibody (ASMA) detected.

Anti-actin autoantibodies are the main component of what have been called Smooth Muscle antibodies (ASMA).

ASMA are found in 52-95% of patients with autoimmune hepatitis (AIH) or chronic active hepatitis (CAH) and in 22% of patients with primary bilary cirrhosis (PBC). ASMA have been reported, usually in low titers, in 3-18% of sera from the general healthy population.