90887:WEST NILE VIRUS ANTIBODY PANEL, CEREBROSPINAL FLUID (CSF)
Author PeaceHealth Laboratories
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| Methodology: |
Enzyme-Linked Immunosorbent Assay (ELISA) |
| Edit Date: |
3/17/2009 |
| Performed: |
Tuesday, Friday |
| Released: |
1-8 days after setup at OML’s reference lab, next run if repeat testing is necessary. |
| CPT Code: |
86788-90 / 86789-90 |
| Specimen Collection Details |
| Collection: |
2 mL cerebrospinal fluid (CSF) in a sterile vial. |
| Handling: |
Specimens must be clearly labeled ‘acute’ or ‘convalescent’. Parallel testing is preferred. Convalescent specimen must be received within 30 days of acute specimen. Avoid repeated freeze/thaw cycles. |
| Stability: |
Specimen stable 8 hours ambient, 14 days refrigerated, or 1 year frozen. |
| Standard Volume: |
2 mL CSF. |
| Minimum Volume: |
0.5 mL CSF. |
| Transport: |
Refrigerated. |
| Comments: |
This test is intended to be used as a semi-quantitative means of detecting West Nile virus-specific IgG and IgM in CSF samples in which there is a clinical suspicion of West Nile virus infection. This test should not be used solely for quantitative purposes, nor should the results be used without correlation to clinical history or other data. Because other members of the Flaviviridae family, such as St. Louis encephalitis virus, show extensive cross-reactivity with West Nile virus, serologic testing specific for these species should also be performed. |
| Rejection Criteria: |
Heat-inactivated, contaminated, or hemolyzed specimen. |
|
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West Nile Virus Antibody, IgG by ELISA, CSF |
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<1.30 IV |
Negative |
No significant level of West Nile virus IgG antibody detected |
|
1.30-1.50 IV |
Equivocal |
Questionable presence of West Nile virus IgG antibody detected. Repeat testing in 10-14 days may be helpful. |
|
>1.50 IV |
Positive |
Presence of IgG antibody to West Nile virus detected, suggestive of current or past infection. |
|
West Nile Virus Antibody, IgM by ELISA, CSF |
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|
|
<.90 IV |
Negative |
No significant level of West Nile virus IgM antibody detected |
|
0.90-1.10 IV |
Equivocal |
Questionable presence of West Nile virus IgM antibody detected. Repeat testing in 10-14 days may be helpful. |
|
>1.10 IV |
Positive |
Presence of IgM antibody to West Nile virus detected, suggestive of current or recent infection |
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