41250:VITAMIN B12

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41250:VITAMIN B12
Alias Names: VIT B12
Methodology: Chemiluminescent Immunoassay (CLIA)
Edit Date: 7/10/2008
Performed: Daily
Released: Same day
CPT Code: 82607
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Fasting specimen recommended. Also acceptable: One 5 mL red top tube or 4 mL green top tube (heparin).
Handling: Allow to clot, centrifuge and separate serum or plasma from cells within 2 hours. Vitamin B12 is a light-sensitive analyte; protect specimen from light. Refrigerate. Freeze if specimen will not be assayed within 48 hours of collection. If specimen is frozen, send a separate specimen if additional testing is ordered. Allow no more than one freeze/thaw cycle.
Stability: 48 hours refrigerated or 30 days frozen.
Standard Volume: 0.5 mL serum or heparinized plasma.
Minimum Volume: 200 µL serum or heparinized plasma.
Transport: Refrigerated, or frozen on dry.
Rejection Criteria: Moderate to gross hemolysis; EDTA specimen; specimen frozen/thawed more than once.

Normal Range 180-914 pg/mL
Indeterminate Range 145-180 pg/mL
Deficient Range <145 pg/mL