97165:VON WILLEBRAND MULTIMERS
Author PeaceHealth Laboratories
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| Alias Names: |
VWF / Multimeric |
| Methodology: |
Westernblot (WB) |
| Edit Date: |
10/29/2010 |
| Performed: |
Sunday, Wednesday |
| Released: |
3-7 days after setup at PeaceHealth Laboratories’ reference lab. |
| CPT Code: |
85247-90 |
| Specimen Collection Details |
| Collection: |
One 4.5 mL light blue top tube (sodium citrate). |
| Handling: |
Within one hour of collection centrifuge and separate plasma. Centrifuge plasma again and separate platelet-poor plasma into a plastic vial. Freeze immediately. CRITICAL FROZEN. |
| Stability: |
14 days frozen. |
| Standard Volume: |
2 mL plasma. |
| Minimum Volume: |
1 mL plasma. |
| Transport: |
Frozen ond dry ice. |
| Comments: |
Useful in differentiating between a normal multimeric pattern, and an abnormal one, where the High Molecular Multimers are missing, such as in type II vWD. It is necessary to test for vWF:Ag as well as a Ristocetin Cofactor at the same time as the multimer analysis if an interpretation of the pattern is needed. |
| Rejection Criteria: |
Hemolysis; microclots. |