40381:ACTH STIMULATION TEST
Author PeaceHealth Laboratories
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| Methodology: |
Chemiluminescent Immunoassay (CLIA) |
| Edit Date: |
9/25/2006 |
| Performed: |
Monday-Friday |
| Released: |
Same day |
| CPT Code: |
80400, 82533 |
| Specimen Collection Details |
| Collection: |
Three specimens total for cortisol levels: one 7.5 mL serum separator tube (SST) per draw. Also acceptable: Plasma separator tube (PST), red top tube or green top tube per draw. Draw baseline specimen prior to injection of cortrosyn. Draw specimens at 30-60minutes following injection (the time interval may vary depending on the physician’s requirements). |
| Handling: |
Allow to clot, centrifuge and separate serum within 2 hours. Refrigerate. Indicate draw time and order of draw, i.e., “1 of 3,” etc., on each specimen. Freeze if specimen cannot be assayed within 48 hours. Allow no more than one freeze/thaw cycle. |
| Standard Volume: |
0.5 mL serum per draw. |
| Minimum Volume: |
100 µL serum per draw. |
| Transport: |
Refrigerated or frozen on dry ice. |
| Comments: |
The procedure is intended to be used as a diagnostic agent in the screening of patients presumed to have adrenocortical insufficiency. |
| Rejection Criteria: |
Specimen frozen and thawed more than once. |
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| See individual components. |