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|80215:WEST NILE VIRUS ANTIBODY, IgG|
|Methodology:||Enzyme-Linked Immunosorbent Assay (ELISA)|
|Released:||1-5 days after setup at PeaceHealth Laboratories’ reference lab|
|Specimen Collection Details|
|Collection:||One 7.5 mL serum separator tube (SST).|
|Handling:||Allow to clot, centrifuge and separate serum from cells immediately and pour into a plastic vial. Refrigerate.|
|Stability:||48 hours ambient, 14 days refrigerated, or 1 year frozen. Allow no more than one freeze/thaw cycle.|
|Standard Volume:||1 mL serum|
|Minimum Volume:||0.5 mL serum|
|Comments:||This test is intended to be used as a semi-quantitative means of detecting West Nile virus-specific IgG in serum specimens in which there is a clinical suspicion of West Nile virus infection.|
|Rejection Criteria:||Plasma; severely lipemic, contaminated, heat-activated, or hemolyzed specimen.|
|≤ 1.29 IV||
Negative – No significant level of West Nile virus IgG antibody detected.
Equivocal – Questionable presence of West Nile virus IgG antibody detected. Repeat testing in 10-14 days may be helpful.
|≥ 1.51 IV||
Positive – Presence of IgG antibody to West Nile virus detected, suggestive of current or past infection.
|Seroconversion between acute and convalescent sera is considered strong evidence of current or recent infection. The best evidence for infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time.|