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|83420:ECHINOCOCCUS ANTIBODY, IgG, SERUM|
|Methodology:||Enzyme-Linked Immunosorbent Assay (ELISA)|
|Released:||1-5 days after setup at PeaceHealth Laboratories’ reference lab|
|Specimen Collection Details|
|Collection:||One 7.5 mL serum separator tube (SST).|
|Handling:||Centrifuge and separate serum from cells immediately. Label specimen clearly as ‘acute’ or ‘convalescent’. Refrigerate.|
|Stability:||4 hours ambient, 72 hours refrigerated, or 1 year frozen (avoid repeated freeze/thaw cycles).|
|Standard Volume:||1 mL serum.|
|Minimum Volume:||150 µL serum.|
|Comments:||Parallel testing is preferred and the convalescent specimen must be received within 30 days from receipt of the acute specimen. Please mark specimens plainly as ‘acute’ or ‘convalescent.’|
|Rejection Criteria:||Plasma; severe lipemia; contaminated specimen.|
|≤ 0.235 OD||
Negative – No significant level of Echinococcus IgG antibody detected.
Equivocal – Questionable presence of Echinococcus IgG antibody detected. Repeat testing in 10-14 days may be helpful.
|≥ 0.300 OD||
Positive – Presence of IgG antibody to Echinococcus detected, suggestive of current or past infection.
|Patients with collagen vascular diseases, hepatic cirrhosis, schistosomiasis, and other parasitic infections can produce false-positive results. There is a strong cross-reaction between echinococcosis and cysticercosis positive sera.
Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time