45260:LECITHIN/SPHINGOMYELIN (L/S) RATIO
Author PeaceHealth Laboratories
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| Alias Names: |
Added L/S |
| Methodology: |
Thin Layer Chromatography (TLC) |
| Edit Date: |
1/27/2010 |
| Performed: |
Daily |
| Released: |
3.5 hours on day tested |
| CPT Code: |
83661 |
| Specimen Collection Details |
| Collection: |
Amniotic fluid. |
| Handling: |
Centrifuge for 3 minutes at 1700 rpm (lower than routine centrifuging). Pour off spun specimen and label container as “Spun.” Refrigerate. Include additional clinical information on requisition, i.e., weeks gestation, is patient diabetic, source if not amniotic fluid, e.g., vaginal pool. |
| Standard Volume: |
5 mL amniotic fluid |
| Minimum Volume: |
2.5 mL amniotic fluid |
| Transport: |
Refrigerated. |
| Comments: |
Visible meconium or blood greater than 2% Hct may interfere with the L/S ratio. |
| Rejection Criteria: |
Mucoid specimen too viscous to pipette. |