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Alias Names: Tissue Plasminogen Activator, Antigen / t-PA / TPA Antigen
Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Edit Date: 5/18/2010
Performed: Wednesday
Released: 1-8 days after setup at PeaceHealth Laboratories’ reference lab
CPT Code: 85415-90
Specimen Collection Details
Collection: One 4.5 mL light blue top tube (sodium citrate).
Handling: Centrifuge at 1700 x g for 15 minutes to produce platelet-poor plasma. Separate plasma from cells and immediately freeze.
Stability: 4 hours ambient, 4 hours refrigerated, or 2 months frozen.
Standard Volume: 1 mL plasma
Minimum Volume: 0.5 mL plasma
Transport: Frozen on dry ice.
Comments: Tissue plasminogen activator (t-PA) plays an important role in the fibrinolytic pathway. The primary function of t-PA is to activate plasminogen to plasmin, which then degrades fibrin to soluble degradation products. There are two forms of t-PA, a single chain and the two-chain form; both forms can activate plasminogen. t-PA is synthesized mainly in the endothelial cells. Typically, t-PA is found in very small amounts. The assessment of t-PA is further complicated by PAI-1 rapidly binding to t-PA, inhibiting its functional activity; thus, to determine active t-PA, acidification of the patient’s plasma is necessary. Excessive t-PA release can lead to excessive activation of fibrinolysis, causing bleeding episodes in patients. Decreased release of t-PA can lead to reduced activation of fibrinolysis, causing thrombosis. The intended use of this ELISA assay is to give a quantitative determination of t-PA in plasma. This assay may be helpful in detecting disorders of the fibrinolytic system.
Rejection Criteria: Serum; hemolysis; plasma from sodium fluoride or heparin tubes.

Reference Range:
0.8-11.2 ng/mL