94530:BRUCELLA TOTAL ANTIBODY AGGLUTINATION
Author PeaceHealth Laboratories
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| Methodology: |
Bacterial Agglutination |
| Edit Date: |
7/28/2010 |
| Performed: |
Monday-Friday |
| Released: |
2-4 days after setup at Peacehealth Laboratories’ reference lab |
| CPT Code: |
86622-90 |
| Specimen Collection Details |
| Collection: |
One 7.5 mL serum separator tube (SST). |
| Handling: |
Allow to clot, centrifuge and immediately separate serum from cells into a plastic vial. Clearly label tube “acute” or “convalescent.” |
| Stability: |
48 hours ambient, 14 days refrigerated, or 1 year frozen. Avoid repeated freeze/thaw cycles. |
| Standard Volume: |
1 mL serum (pediatric: 200µL) |
| Minimum Volume: |
0.5 mL serum |
| Transport: |
Refrigerated. |
| Comments: |
Parallel testing is preferred and convalescent specimen MUST be received within 30 days from receipt of acute specimen. Cross reactions may occur between Brucella and F. tularensis antigens and antisera; therefore, parallel tests should be run with those antigens. A fourfold rise in titer ≥ 1:160 is considered diagnostic. A single serum titer of 1:160 or 1:320 is suggestive of Brucellosis when accompanied by a compatible clinical course in a patient with a history of potential exposures. |
| Rejection Criteria: |
Severely lipemic, contaminated, hemolyzed or heat inactivated specimen. |