45150:FETAL LUNG MATURITY CASCADE

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45150:FETAL LUNG MATURITY CASCADE
Methodology: S/A Ratio: Fluorescent Polarization Immunoassay (FPIA); L/S & PG: Thin Layer Chromatography with Cupric Acetate Charring
Edit Date: 1/3/2007
Performed: Daily at 1300 (STAT 800)
Released: Results at 1700 (STAT at 1300)
CPT Code: 83663
Specimen Collection Details
Collection: 7.5 mL amniotic fluid.
Handling: Pour off minimum 1.5 mL unspun amniotic fluid and label ‘unspun.’ Centrifuge remainder of specimen for 3 minutes at 1700 rpm (lower than routine centrifuging). Transfer supernatant to a plastic screw-top vial and label ‘spun.’ Refrigerate. Include additional clinical information on requisition, i.e., weeks gestation, is patient diabetic, source if not amniotic fluid, e.g., vaginal pool.
Stability: 24-36 hours refrigerated or 14 days frozen.
Standard Volume: 7.5 mL amniotic fluid.
Minimum Volume: 1 mL amniotic fluid for S/A Ratio, 2.5 mL for L/S Ratio and 1.5 mL for PG.
Transport: Refrigerated, or frozen on dry ice if transport is delayed.
Comments: Visible meconium or blood greater than 2% Hct may interfere with the S/A and L/S ratio.

The S/A ratio is done first and resulted within 45 minutes after specimen receipt in department. If S/A ratio is mature, testing stops; the L/S and PG will not be performed. If the S/A ratio is immature, L/S and PG testing will be performed on the next run.

Rejection Criteria: Vaginal specimen too mucoid to pipette.