21400:HEMOGLOBIN S, SICKLE CELL EXAM
Author PeaceHealth Laboratories
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| Alias Names: |
Sickle Cell Exam |
| Methodology: |
Sodium metabisulfite sickling test |
| Edit Date: |
3/17/2009 |
| Performed: |
Daily |
| Released: |
Next day |
| CPT Code: |
85660 |
| Specimen Collection Details |
| Collection: |
One 4 mL lavender top tube (EDTA). Fill tube at least 1/3 full. |
| Handling: |
Refrigerate. Send to the laboratory within 24 hours. |
| Standard Volume: |
4 mL EDTA whole blood. |
| Minimum Volume: |
0.5 mL EDTA whole blood in a 1 mL microcollection tube. |
| Transport: |
Refrigerated. |
| Comments: |
Sodium metabisulfite-induced sickling of red cells containing hemoglobin S. The presence or absence of sickling is observed microscopically. This test becomes positive at Hgb S levels equal to or greater than 25%. Thus it is possible for a negative result to occur in some combined defects such as Hbg S accompanied by α or β thalassemia. For alternative or additional tests, see Hemoglobin S Quantitation (48830), or Hemoglobinopathy Evaluation (10570) . |
| Rejection Criteria: |
Frozen specimen. |