21400:HEMOGLOBIN S, SICKLE CELL EXAM

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21400:HEMOGLOBIN S, SICKLE CELL EXAM
Alias Names: Sickle Cell Exam
Methodology: Sodium metabisulfite sickling test
Edit Date: 3/17/2009
Performed: Daily
Released: Next day
CPT Code: 85660
Specimen Collection Details
Collection: One 4 mL lavender top tube (EDTA). Fill tube at least 1/3 full.
Handling: Refrigerate. Send to the laboratory within 24 hours.
Standard Volume: 4 mL EDTA whole blood.
Minimum Volume: 0.5 mL EDTA whole blood in a 1 mL microcollection tube.
Transport: Refrigerated.
Comments: Sodium metabisulfite-induced sickling of red cells containing hemoglobin S. The presence or absence of sickling is observed microscopically. This test becomes positive at Hgb S levels equal to or greater than 25%. Thus it is possible for a negative result to occur in some combined defects such as Hbg S accompanied by α or β thalassemia. For alternative or additional tests, see Hemoglobin S Quantitation (48830), or Hemoglobinopathy Evaluation (10570) .
Rejection Criteria: Frozen specimen.

No sickle cells seen.