45250:LECITHIN/SPHINGOMYELIN (L/S) RATIO

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45250:LECITHIN/SPHINGOMYELIN (L/S) RATIO
Alias Names: L/S RATIO
Methodology: Thin Layer Chromatography (TLC)
Edit Date: 1/3/2007
Performed: Daily at 1300 (STAT 800)
Released: Results at 1700 (STAT at 1300)
CPT Code: 83661
Specimen Collection Details
Collection: 5 mL amniotic fluid.
Handling: Centrifuge for 3 minutes at 1700 rpm (lower than routine centrifuging). Transfer supernatant to a plastic, screw-cap vial and label ”spun.” Refrigerate. Include additional clinical information on requisition, i.e., weeks gestation, is patient diabetic, source if not amniotic fluid, e.g., vaginal pool.
Standard Volume: 5 mL amniotic fluid.
Minimum Volume: 2.5 mL amniotic fluid.
Transport: Refrigerated.
Comments: Visible meconium or blood greater than 2% Hct may interfere with the L/S ratio.
Rejection Criteria: Mucoid samples too viscous to pipette.

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