27020:MIXING STUDY, PROTHROMBIN TIME

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27020:MIXING STUDY, PROTHROMBIN TIME
Alias Names: Prothrombin Time, Mixing Study
Methodology: Mechanical Clot Detection
Edit Date: 6/6/2008
Performed: Monday-Friday (STAT only on weekends)
Released: Next day
CPT Code: 85611 x3
Specimen Collection Details
Collection: Three 4.5 mL light blue top tubes (sodium citrate). Collect by careful venipuncture to avoid tissue contamination or hemolysis. Do not use needles smaller than 23 gauge. Fill tubes to maximum draw (at least 90% full). Mix by gentle inversion.
Handling: Due to short stability, specimens must either be sent STAT to arrive in testing department within 4 hours, or sent frozen. If STAT, refrigerate and keep capped in original tubes. Call for STAT pick-up. For standard delivery, centrifuge to produce platelet poor plasma and with a plastic pipette, transfer into a plastic vial. Label with patient name, I.D. number, test, and “citrated plasma.” Freeze immediately. Send to PeaceHealth Laboratories within 7 days. If multiple tests are ordered, submit a separate aliquot for this test.
Stability: 4 hours ambient or refrigerated, or 7 days frozen.
Standard Volume: Three 4.5 mL tubes whole blood or 4 mL plasma.
Minimum Volume: Two 2.7 mL tubes whole blood or 2 mL plasma.
Transport: Original tubes refrigerated if sent STAT; plasma frozen on dry ice.
Comments: See also: Coagulation Study, Prolonged Protime Evaluation (25720).
Rejection Criteria: Visible fibrin; moderate to marked hemolysis; normal Prothrombin Time; specimen received in testing department with 4 hours of collection.

Reference Range:
Normal = Negative for coagulation inhibitor. Pathologist interpretation accompanies report.