21600:OSMOTIC FRAGILITY TEST, RBC

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21600:OSMOTIC FRAGILITY TEST, RBC
Alias Names: RBC / Spherocytosis Test, RBC
Methodology: Spectrophotometry
Edit Date: 1/21/2008
Performed: Daily
Released: 48 hours
CPT Code: 85557
Specimen Collection Details
Collection: Two 4 mL lavender top tubes (EDTA). If possible, collect Monday-Thursday. Also acceptable: Heparinized blood may be tested as an alternative specimen, but must be received in the laboratory within 2 hours of collection.
Handling: Prepare two blood films and submit with the blood. Send to PeaceHealth Laboratories within 4 days of collection.
Stability: 4 days refrigerated.
Standard Volume: 4 mL EDTA whole blood.
Minimum Volume: 2 mL EDTA whole blood.
Transport: Refrigerated.
Comments: For the confirmation of peripheral blood spherocytosis. Testing is not recommended in children less than 1 year old, as reference ranges and clinical interpretation have not been well studied for this age group. Testing of parents may be performed, but is only helpful if positive. A negative test in both parents does not completely exclude hereditary spherocytosis in their children.
Rejection Criteria: Clotting; marked hemolysis; frozen specimen.

Pathologist interpretation accompanies report.