10780:ANTINUCLEAR ANTIBODIES (ANA) REFLEX PROFILE
Author PeaceHealth Laboratories
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| Methodology: |
Multiple Flow Immunoassay / Nephelometry (NEPH) |
| Edit Date: |
6/11/2010 |
| Components: |
Complement C3 / Complement C4 / Double Stranded DNA Antibody (DS DNA) / Extractable Nuclear Antigen (ENA) Antibodies, SM & RNP / Extractable Nuclear Antigen (ENA) Antibodies, SS-A/Ro & SS-B/La |
| Performed: |
Daily |
| Released: |
Same day as tested |
| CPT Code: |
86225 / 86160 x2 / 86235 x4 |
| Specimen Collection Details |
| Collection: |
Two 7.5 mL serum separator tubes (SST). Also acceptable: Two 5 mL red top tubes. |
| Handling: |
Allow to clot for 30-60 minutes at 20-25° C. Do not clot at 2-8° C. Centrifuge and within 1 hour separate serum into two plastic vials. For C3/C4 Testing: Freeze one tube ≤ -20°C. Allow no more than one freeze/thaw cycle. For ANA Testing: Refrigerate second tube at 2-8° C. Freeze ≤ -20° C if specimen cannot be tested within 7 days. Allow no more than three freeze/thaw cycles. |
| Stability: |
C3/C4: 1 year frozen ≤ -20° C; ANA: 7 days refrigerated or frozen ≤ -20°C for long-term storage. |
| Standard Volume: |
1 mL serum for ANA testing and 1 mL serum for C3/C4 testing |
| Minimum Volume: |
0.5 mL serum for ANA testing and 0.5 mL serum for C3/C4 testing. |
| Transport: |
C3/C4: Frozen on dry ice. ANA: Refrigerated or frozen on dry ice. |
| Rejection Criteria: |
C3/C4: Plasma; grossly lipemic, hemolytic, or contaminated specimen; specimen allowed to clot at 2-8° C; specimen frozen/thawed more than once. ANA: Plasma; grossly lipemic, hemolytic, or contaminated specimen; refrigerated specimen received in lab more than 7 days after collection; specimen frozen thawed more than 3 times |
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| See individual components. |