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|89220:VITAMIN B1 PYROPHOSPHATE (THIAMINE). WHOLE BLOOD|
|Alias Names:||B1 / VIT B1|
|Methodology:||High Performance Liquid Chromatography (HPLC) for Thiochrome|
|Released:||2-6 days after setup at PeaceHealth Laboratories’ reference lab.|
|Specimen Collection Details|
|Collection:||One 4 mL green top tube (heparin). Do NOT use a plasma separator tube. Also acceptable: One 4 mL lavender top tube (EDTA) or 6 mL pink top tube (K2 EDTA). Protect from light during collection, storage, and transport.|
|Handling:||Immediately transfer whole blood to a plastic vial and freeze. CRITICAL FROZEN. Protect from light. If multiple tests are ordered, send separate specimen for this test.|
|Stability:||4 hours refrigerated or 6 months frozen. Specimen unstable ambient.|
|Standard Volume:||3 mL whole blood.|
|Minimum Volume:||0.6 mL whole blood.|
|Transport:||Frozen on dry ice.|
|Rejection Criteria:||Specimen not frozen within 4 hours of collection; serum or plasma; gross lipemia; use of a PST; specimen exposed to light or submitted in glass tube; non-frozen or thawed specimen.|
The concentration of thiamine diphosphate (TDP), the primary active form of vitamin B1, is measured in this assay. Approximately 90% of vitamin B1 present in whole blood is TDP. Thiamine and thiamine monophosphate, which comprise the remaining 10%, are not measured.
Note: Whole blood is the preferred specimen for thiamine assessment. Approximately 80% of thiamine present in whole blood is found in red blood cells. This assay does not distinguish thiamine from its phosphate esters.