29100:FLOW CYTOMETRY, T-CELL SUBSETS

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29100:FLOW CYTOMETRY, T-CELL SUBSETS
Alias Names: CD4 & CD8 Lymphocytes / T-Cell Subsets
Methodology: Flow Cytometry (FC)
Edit Date: 2/12/2008
Components: Total T Lymphocytes (CD3) / T-Helper Lymphocytes (CD4) / T Suppressor Lymphocytes (CD8)
Performed: Daily
Released: 24 hours
CPT Code: 86359 / 86360
Specimen Collection Details
Collection: One 4 mL lavender top tube (EDTA).
Handling: Submit specimens to the laboratory within 48 hours. If specimens will not be received in the laboratory within 24 hours, perform a CBC and submit results with specimen at ambient temperature or refrigerated.
Stability: 48 hours ambient or refrigerated. Unstable frozen.
Standard Volume: 4 mL whole blood.
Minimum Volume: 1 mL whole blood.
Transport: Ambient or refrigerated.
Comments: Provide patient’s Social Security Number and date of birth to assure correct matching of patient for cumulative historical reports. NOTE: for evaluation of possible lymphoma, CLL or other lymphoproliferative disorders see Flow Cytometry, Lymphoid Panel (29400).
Rejection Criteria: Clotted or frozen specimen; specimen more than 48 hours old at time of testing.

Relative Count Absolute Count
CD3 62.0-85.0% 850-2560/uL Total T Lymphocytes
CD4 39.0-64.0% 680-1760/uL T-Helper Lymphocytes
CD8 16.0-37.0% 200-900/uL T Suppressor/Cytotoxic lymphs
CD4/8 Ratio 1.0-3.2