87080:ALKALINE PHOSPHATASE ISOENZYMES

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87080:ALKALINE PHOSPHATASE ISOENZYMES
Alias Names: Phosphatase Isoenzymes
Methodology: Heat Inactivation / Enzymatic
Edit Date: 6/11/2010
Performed: Daily
Released: 24-72 hours after setup at PeaceHealth Laboratories’ reference lab.
CPT Code: 84080-90
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 4 mL green top tube (heparin). Fasting specimen is recommended.
Handling: Allot to clot, centrifuge and separate serum from cells immediately and pour into a plastic vial. Freeze. Allow no more that one freeze/thaw cycle.
Stability: After separation specimen stable 1 hour ambient, 7 days refrigerated (total activity will increase 2% per day), or 1 year frozen. Allow no more than one freeze/thaw cycle.
Standard Volume: 2 mL serum or plasma.
Minimum Volume: 1 mL serum or plasma.
Transport: Frozen on dry ice.
Comments: This test cannot be added on at a later date due to stability.

Heat-stable isoenzymes, such as the one produced during pregnancy, will interfere with the interpretation of this assay.

Rejection Criteria: EDTA, sodium fluoride/potassium oxalate plasma, or hemolyzed specimen.

Bone Male Female
1-6 years:0-208 U/L 1-6 years: 0-189 U/L
7-9 years: 0-264 U/L 7-9 years: 0-246 U/L
10-15 years: 0-340 U/L 10-13 years: 0-340 U/L
16-19 years: 0-165 U/L 14-15 years: 0-91 U/L
20 years >:0-55 U/L 16 years >: 0-55 U/L
Liver 1-6 years: 0-145 U/L 1-9 years: 0-148 U/L
7-11 years: 0-182 U/L 10-15 years: 0-162 U/L
12-15 years: 0-226 U/L 16 years >: 0-94 U/L
16-19 years: 0-114 U/L
20 years >: 0-94 U/L
Alkaline Phosphatase, Serum or Plasma Age Male Female
0-30 days 60-320 U/L 60-320 U/L
1-12 months 70-350 U/L 70-350 U/L
1-3 years 125-320 U/L 125-320 U/L
4-6 years 125-320 U/L 150-370 U/L
7-9 years 150-370 U/L 150-440 U/L
10-11 years 150-470 U/L 150-530 U/L
12-13 years 160-500 U/L 110-525 U/L
14-15 years 130-530 U/L 55-305 U/L
16-19 years 60-270 U/L 40-120 U/L
20> years 40-120 U/L 40-120 U/L