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Alias Names: H. Pylori / H Pylori
Methodology: Enzyme-Linked Immunosorbent Assay (ELISA)
Edit Date: 4/19/2010
Performed: Tuesday, Thursday
Released: Same day as tested
CPT Code: 86677 x2
Specimen Collection Details
Collection: One 7.5 mL serum separator tube (SST). Also acceptable: One 5 mL red top tube.
Handling: Allow to clot, centrifuge and immediately separate serum from cells into a plastic vial. Refrigerate. Freeze -20° C if not assayed within 72 hours. Allow no more than one freeze/thaw cycle.
Stability: 72 hours refrigerated.
Standard Volume: 0.5 mL serum.
Minimum Volume: 200 µL serum.
Transport: Refrigerated, or frozen on dry ice.
Rejection Criteria: Plasma; gross lipemia; gross hemolysis; bacterial contamination; specimen frozen and thawed more than once; heat-inactivated specimen.

Helicobacter Pylori IgG and IgA Antibodies Result Interpretations, ELISA Method:
0.88 IV
<0.89 IV
Negative: No significant level of H. Pylori IgG and IgA antibody detected.
0.89-0.99 IV
Equivocal: Repeat testing in 10-14 days may be helpful.
>=1.00 IV
Positive: IgG antibody to H. Pylori suggests previous exposure or active infection. IgA antibody to H. Pylori suggests active infection.
IV = Index Value
Helicobacter Pylori IgG and IgA antibody seroconversion occur together after 60 days. Samples which have a high titer of both IgG and IgA antibodies to H. Pylori in symptomatic individuals may be considered to represent an active infection. However, a positive H. Pylori IgA result can only infer active infection and should be confirmed by bacterial isolation or other diagnostic testing.

Helicobacter Pylori antibodies are detectable in almost all adult patients with duodenal ulcer and about 80% of patients with gastric ulcer. The prevalence of H. Pylori antibodies increases with age and can be found in a significant percentage of healthy individuals over the age of 50. Positive results do not confirm the diagnosis of H. Pylori-associated gastritis or duodenal ulcer, but negative results are strong evidence against these diagnoses.