81670:BORRELIA BURGDORFERI, IgG & IgM BY EIA and IB
Author PeaceHealth Laboratories
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| Alias Names: |
B. Burgdorferi / Lyme Disease |
| Methodology: |
Enzyme Immunoassay (EIA) / Immunoblot (IB) |
| Edit Date: |
3/12/2008 |
| Performed: |
Tuesday-Saturday |
| Released: |
1-5 days after setup at PeaceHealth Laboratories’ reference lab. |
| CPT Code: |
86617-90 x2 / 86618-90 x2 |
| Specimen Collection Details |
| Collection: |
One 7.5 mL serum separator tube (SST). |
| Handling: |
Allow to clot, centrifuge and separate serum from cells and pour into a plastic vial. |
| Stability: |
7 days ambient , 14 days refrigerated, or 2 months frozen. |
| Standard Volume: |
1 mL serum. |
| Minimum Volume: |
0.5 mL serum. |
| Transport: |
Refrigerated. |
| Comments: |
Following the two-tier test algorithm proposed by the CDC for assessment of exposure to B. burgdorferi during early- and late-stage disease, both EIA IgM and IgG antibody responses to B. burgdorferi in human serum, are confirmed using an immunoblot (IB) method where whole-cell sonicate (WCS) B. burgdorferi antigens are utilized in identifying B. burgdorferi specific antigen binding reactivity. There are multiple criteria for interpreting Lyme immunoblots. The CDC recommends that at least 2 of 3 IgM bands (23, 39, or 41 kDa) must be present for interpretation of an IgM positive blot. For interpretation of an IgG positive blot, at least 5 of 10 IgG bands (18, 23, 28, 30, 39, 41, 45, 58, 66, or 93kDa) must be present. Only positive and negative interpretations are reported.
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Component |
Reference Range |
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Borrelia burgdorferi IgG Antibodies – IB |
Negative |
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Borrelia burgdorferi IgM Antibodies – IB |
Negative |
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Borrelia burgdorferi IgG Antibodies – EIA |
< .80 Index |
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Borrelia burgdorferi IgM Antibodies – EIA |
< .80 Index |
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